An Understanding Of Bacterial Transformation By Plasmid Dna

Bacterial Transformation Lab Report Elizabeth Amanquah Genetics 311 Monday 2:00-4:50 Jan Trybula Abstract This study was undertaken to develop an understanding of bacterial transformation by plasmid DNA. This study enables one to observe various traits displayed by transformed bacterial cells. Four experiments were conducted that included (1) Bacterial Transformation, (2) Genomic/Plasmid extraction, (3) DNA Electrophoresis, and(4) Plasmid /Oxidation EMSA. In this study, a transformation of the strain Escherichia coli also known as E. coli will be examined with a special plasmid called pGLO. This study was done to prepare the plasmid into a bacterium and use it to make large quantities by amplifying it. By allowing RNA†¦show more content†¦The genetic material that is being incorporated into is the bacterial plasmid In genetic transformation, DNA genes of one organism are added to another organism. The DNA that has been transmitted to the new recipient of the genetic material is then expressed and continued (Holmes et al. 1996). By doing this, large quantities of preferable genes can be made. The process of transformation can be done naturally or artificially. Escherichia coli is a common bacterium this is single celled allowing the process for plasmid exchange to not be difficult. The bacterium produces clones allowing the DNA to not be mixed. A plasmid is a circular deoxyribonucleic acid (DNA) molecule that is able to replicate itself of the bacterial chromosome and is often the gateway for which a bacteria is able to gain resistant to an antibiotic. Plasmid contains the promoter of the arabinose operon called PBAD, which is a functioning unit of genomic DNA containing a collection of genes under the control of a single promoter. The genes are copied into RNA together into an mRNA strand to encode a single gene product. The plasmid DNA will be used integrated into the genome of the E. coli bacteria. To generate the effects of the plasmids and transformation, the